The addition of cold ethanol eliminates water molecules from the
DNA, and the pellets of the DNA molecules are precipitated in the
area between water-ethanol, because the DNA is not soluble
in ethanol. The alcohol is added to the frozen because the solubility
of the salts in liquids decreases with falling temperature.
5. The cold extraction buffer retards enzymatic reactions and
protects the DNA by the action of nucleases are
most free fluid (after cleavage of the vesicles containing them
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